王玉俭;代绍密;李晓燕;黄冰冰;宋冠华;钟平生;

• 1:惠州学院生命科学学院

摘要(Abstract)：

为探究长角血蜱（Haemaphysalis longicornis）巨噬细胞移动抑制因子（Macrophage migration inhibitory factor,MIF）基因（HlMIF）的功能，采用RT-PCR方法对HlMIF基因进行扩增，运用大肠杆菌表达体系对HlMIF基因进行体外重组表达，随后对纯化后的重组HlMIF蛋白进行互变异构梅活性分析。结果表明：HlMIF基因开放阅读框为351 bp，编码116个氨基酸，预测成熟蛋白的分子量为12.5 ku;HlMIF基因在大肠杆菌上清中成功表达，大小为17.4ku；重组HlMIF蛋白具有互变异构酶活性；利用大肠杆菌成功表达具有酶活力的重组HlMIF蛋白，为进一步明确该蛋白的生物学功能提供条件。

关键词(KeyWords)： 长角血蜱;巨噬细胞移动抑制因子;原核表达;互变异构酶活性

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目（32102700）;; 广东省基础与应用基础研究基金区域联合基金项目（2020A1515110318）

作者(Authors): 王玉俭;代绍密;李晓燕;黄冰冰;宋冠华;钟平生;

DOI: 10.16778/j.cnki.1671-5934.2022.06.006

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