银叶树内生黑孢菌HU0460抗细菌活性成分研究Antibacterial Metabolites of Endophytic Nigrospora sp. HU0460 from Heritiera littoralis
叶玉珍;钟志娟;李馨;钟富丹;黄钰涵;毛露甜;徐良雄;
摘要(Abstract):
植物内生真菌次生代谢产物资源丰富,是发现新型抗生素的重要来源。在活性追踪的指导下,从银叶树内生黑孢菌HU0460的玉米发酵物中分离鉴定了4个蒽醌类化合物,分别为化合物(1、2、3和4):4a-epi-4β,9α-dimethoxydihydrodeoxybostrycin(1)、4a--epi--9α-methoxydihydrodeoxybostrycin(2)、9α-hydroxyhalorosellinia A(3)和bostrycin(4),其中第1个为新化合物。化合物4对金黄色葡萄球菌、黑胫病菌和青枯病菌的抗菌活性较高,其MIC分别为25、50和12.5μg/mL,其余3个化合物对上述测试菌表现出较弱的抑制活性,所有化合物对大肠杆菌无明显的抑制活性。研究结果初步阐明该菌的抗菌活性物质基础,对黑孢菌属抗菌成分的深入研究与开发方面具有一定的参考价值。
关键词(KeyWords): 黑孢菌属;蒽醌;分离纯化;抗细菌活性
基金项目(Foundation): 国家自然科学基金项目(31772032);; 广东省大学生创新训练项目(S202110577048);; 广东省科技创新战略专项资金项目(pdjh202160481)
作者(Authors): 叶玉珍;钟志娟;李馨;钟富丹;黄钰涵;毛露甜;徐良雄;
DOI: 10.16778/j.cnki.1671-5934.2022.06.007
参考文献(References):
- [1]MANSFIELD J,GENIN S,MAGORI S,et al. Top 10 plant pathogenic bacteria in molecular plant pathology. Molecular Plant Pathology[J]. 2012,13(6):614-629.
- [2]杨松,胡林双,吕文河,等.实时定量荧光PCR法检测马铃薯黑胫病菌[J].中国马铃薯,2009,23(1):1-5.
- [3]洪小兰,周少波,凌月明,等.金黄色葡萄球菌感染的临床分布及耐药性分析[J].临床合理用药杂志,2022,15(13):15-18.
- [4]胡付品,郭燕,朱德妹,等. 2018年CHINET中国细菌耐药性监测[J].中国感染与化疗杂志,2020,20(1):1-10.
- [5]孙双勇,王蒙蒙,李丽,等.抗生素耐药与抗生素新药开发的研究进展[J].现代药物与临床,2022,37(2):221-229.
- [6]PORRAS G,CHASSAGNE F,LYLES J T,et al. Ethnobotany and the role of plant natural products in antibiotic drug discovery[J].Chemical Reviews,2021,121(6):3495-3560.
- [7]卜宣尹,杨卫丽.植物内生菌抑菌机制和抑菌次生代谢产物的研究进展[J].现代药物与临床,2021,36(10):2200-2206.
- [8]常东东,戴好富,左文健,等.海南龙血树内生真菌A12中的抗菌活性成分研究[J].中国抗生素杂志,2012,37(6):421-424,448.
- [9]UBONTA S,VATCHARIN R U,YAOWAPA S,et al. Hydronaphthalenones and a dihydroramulosin from the endophytic fungus PSU-N24[J]. Chemical&Pharmaceutical Bulletin,2008,56(12):1687-1690.
- [10]YANG K L,WEI M Y,SHAO C L,et al. Antibacterial anthraquinone derivatives from a sea anemone-derived fungus Nigrospora sp.[J]. Journal of Natural Products,2012,75(5):935-941.
- [11]CHEN H,ZHANG L L,LONG Y H,et al. Studies on the synthesis of derivatives of marine-derived bostrycin and their structureactivity relationship against tumor cells[J]. Marine Drugs,2012,10(12):932-952.
- [12]童佳颖,张鑫,李娇,等. Nigrospora属真菌化学成分及生物活性研究进展[J].天然产物研究与开发,2022,34(9):1618-1331.
- [13]UKWATTA K M. The study of antimicrobial,anti-cancer,anti-inflammatory and α-glucosidase inhibitory activities of nigronap thaphenyl,isolated from an extract of Nigrospora sphaerica[J]. Mycology,2019,10(4):222-228.
- [14]TRISUWAN K,RUKACHAISIRIKUL V,SUKPONDMA Y,et al. Pyrone derivatives from the marine-derived fungus Nigrospora sp. PSU-F18[J]. Phytochemistry,2009,70(4):554-557.
- [15]WANG C,WANG J,HUANG Y H,et al. Anti-mycobacterial activity of marine fungus-derived 4-deoxybostrycin and nigrosporin[J]. Molecules,2013,18:1728-1740.
- [16]XIA X K,LI Q,LI J,et al. Two new derivatives of griseofulvin from the mangrove endophytic fungus Nigrospora sp.(Strain No.1403)from Kandelia candel(L.)Druce[J]. Planta Medica,2011,77(15):1735-1738.
- [17]TRISUWAN K,KHAMTHONG N,RUKACHAISIRIKUL V,et al. Anthraquinone,cyclopentanone,and naphthoquinone derivatives from the sea fan-derived fungi Fusarium spp. PSU-F14 and PSY-F135[J]. Journal of Natural Products,2010,73(9):1507-1511.
- [18]ZhANG H Q,DENG Z S,GUO Z Y,et al. Anthraquinone metabolites from endophytic Nigrospora sp. BM-2 and optimization of fermentation medium for high bostrycin production[J]. Journal of Pure&Applied Microbiology,2013,7(4):2489-2494.